Wolbachia Katzir (Ori Hillel , Liran Shemen) 2

February 4, 2025

Sample information
Picture
Photos by: Liran Shemen
Location
Collection date 12/25/2024
Captive / Cultivated? Wild-caught
Group Katzir High School
Observations

Caught on the wall
Putative identification Arthropoda Insecta Diptera

Methods
Extraction kit DNeasy (Qiagen)
DNA extraction location Whole arthropod
Single or Duplex PCR Single Reaction
Gel electrophoresis system MiniOne
Buffer TAE
DNA stain Other
Gel images
Protocol notes

1. Caught the bug with our hands
2. Place it in a Ethanol-filled test tube
3. DNA extraction process
4. DNA replication using PCR machine
5. electrophoresis

Results
Wolbachia presence Yes
Confidence level Low
Explanation of confidence level

There was a small and faint band which we assume is Wolbachia, probably in a small amount
Wolbachia 16S sequence
Arthropod COI sequence Download FASTA    Download AB1
NNNNNNAANNTNNNNTTTCNNNGCTTGATCCGGAATAGTAGGAACATCATTAAGTATTTTAATTCGAGCTGAATTAGGAC ATCCTGGTGCTTTAATTGGTGATGACCAAATTTATAATGTAATTGTTACTGCTCATGCATTTATTATAATTTTTTTTATA GTTATACCAATTATAATTGGAGGATTTGGAAATTGACTAGTCCCTTTAATATTAGGAGCCCCAGATATAGCCTTTCCTCG AATGAATAATATAAGTTTCTGACTTTTACCACCAGCTTTAACTTTACTACTAGCCAGTAGTATAGTGGAAAACGGAGCTG GAACTGGTTGAACAGTTTACCCTCCTCTATCATCTAATATCGCTCATGGAGGAGCTTCTGTTGATTTAGCTATTTTTTCA CTACATTTAGCAGGAATTTCTTCAATTTTAGGAGCTGTAAATTTTATTACTACTGTAATTAATATACGATCAACAGGAAT TACTTTCGATCGAATACCTTTATTTGTATGATCAGTAGCTATTACAGCTCTATTATTATTATTATCTTTACCTGTATTAG CAGGAGCTATTACAATATTATTAACAGATCGAAATTTAAATACTTCTTTTTTCGACCCTGCTGGAGGAGGAGACCCAATT TTATATCAACACTTATTTTGATTTTTTGGTCACCCTGAAGTTTAA
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Diptera was found to be postive for Wolbachia.
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