Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 02/14/2025 |
| Captive / Cultivated? | Captive / Cultivated |
| Group | John Overton High School |
| Observations | Specimen was acquired through Bugsincyberspace.com, so the exact place of origin is unknown. |
| Putative identification | Arthropoda Insecta Coleoptera Carabidae Brachinus Brachinus nigriventris |
Methods |
|
| Extraction kit | DNeasy (Qiagen) |
| DNA extraction location | Abdomen |
| Single or Duplex PCR | Duplex Reaction |
| Gel electrophoresis system | MiniPCR |
| Buffer | TAE |
| DNA stain | SYBR Safe |
| Gel images |
|
| Protocol notes | Arthropod debris was present during cell lysis, resulting in fragments of exoskeleton represented in the gel smudges. |
Results |
|
| Wolbachia presence | Unknown |
| Confidence level | Low |
| Explanation of confidence level | Results were inconclusive due to smudges on the gel. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | |
Common Eastern Bumble Bee (Bombus impatiens)
American Bird
Spotted crane fly
Wolbachia data
Meadow Katydid