Rachel S. Fruit Fly

April 1, 2025

Sample information
Picture
Photos by: Rachel S.
Location
Collection date 02/18/2025
Captive / Cultivated? Captive / Cultivated
Group Berkshire Community College
Observations

The fruit fly is from a captive collection from the Berkshire Community College Campus in Pittsfield, MA. The fruit fly has red eyes, transparent wings.
Putative identification Arthropoda Insecta Diptera

Methods
Extraction kit Monarch DNA extraction (NEB)
DNA extraction location Whole arthropod
Single or Duplex PCR Single Reaction
Gel electrophoresis system Edvotek Gel Electrophoresis
Buffer TAE
DNA stain SYBR Safe
Gel images
Protocol notes

For the DNA extraction we followed the protocol with no issues. The 1.5ml tubes were in the incubator for 37 minuets. We followed the insect adaptation of the protocol.

Results
Wolbachia presence Yes
Confidence level High
Explanation of confidence level

We have a high confidence level. The four control lanes matched our expectations. The positive controls look as they should because they have a bight band at 438bp. The negative controls showed no bands.
Wolbachia 16S sequence Download AB1
TGGCTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTCATCCTTAGTTACCATCAGG TAATGCTGGGGACTTTAAGGAAACTGCCAGTGATAAACTGGAGGAAGGTGGGGATGATGTCAAGTCATCATGGCCCTTAT GGAGTGGGCTACACACGTGCTACAATGGTGGCTACAATGGGCTGCAAAGTCGCGAGGCTAAGCTAATCCCTTAAAAGCCA TCTCAGTTCGGATTGTACTCTGCAACTCGAGTGCATGAAGTTGGAATCGCTAGTAATCGTGGATCAGCACGCCACGGTGA ATACGTTCTCGGGTCTTGTACACACTGCCCGTCACGCCATGGGAATTGG
BLAST at The Wolbachia Project   BLAST at NCBI
Arthropod COI sequence Download AB1
AGTTGGAACATCTTTAAGAATTTTAATTCGAGCTGAATTAGGACATCCTGGAGCATTAATTGGAGATGATCAAATTTATA ATGTAATTGTAACTGCACATGCTTTTATTATAATTTTTTTTATAGTTATACCTATTATAATTGGTGGATTTGGAAATTGA TTAGTGCCTTTAATATTAGGTGCTCCTGATATAGCATTCCCACGAATAAATAATATAAGATTTTGACTTCTACCTCCTGC TCTTTCTTTACTATTAGTAAGTAGAATAGTTGAAAATGGAGCTGGGACAGGATGAACTGTTTATCCACCTCTATCCGCTG GAATTGCTCATGGTGGAGCTTCAGTTGATTTAGCTATTTTTTCTCTACATTTAGCAGGAATTTCTTCAATTTTAGGAGCT GTAAATTTTATTACAACTGTAATTAATATACGATCAACAGGAATTTCATTAGATCGTATACCTTTATTTGTTTGATCAGT AGTTATTACTGCTTTATTATTATTATTATCACTTCCAGTACTAGCAGGAGCTATTACTATATTATTAACAGATCGAA
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Diptera was found to be postive for Wolbachia.
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