Mosquito – arthropod 2

April 1, 2025

Sample information
Picture
Photos by: Ahmed, Kaleb, Jugraj
Location
Collection date
Captive / Cultivated? Wild-caught
Group Penn State Lehigh Valley
Observations

found in the landscape
Putative identification Arthropoda Insecta Diptera Culicidae

Methods
Extraction kit DNeasy (Qiagen)
DNA extraction location Whole arthropod
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system Bio-Rad
Buffer TAE
DNA stain GelRed
Gel images
Protocol notes

the petri dish in the photo above was mislabeled. this one is supposed to be arthropod 2 or (insect 2)

Results
Wolbachia presence No
Confidence level Medium
Explanation of confidence level
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Culicidae was found to be negative for Wolbachia.
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