Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | |
| Captive / Cultivated? | Wild-caught |
| Group | Penn State Lehigh Valley |
| Observations | found in the landscape |
| Putative identification | Arthropoda Hexapoda Insecta Diptera Culicidae |
Methods |
|
| Extraction kit | DNeasy (Qiagen) |
| DNA extraction location | Whole arthropod |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | Standard electrophoresis system Bio-Rad |
| Buffer | TAE |
| DNA stain | GelRed |
| Gel images |
|
| Protocol notes | the petri dish in the photo above was mislabeled. this one is supposed to be arthropod 2 or (insect 2) |
Results |
|
| Wolbachia presence | No |
| Confidence level | Medium |
| Explanation of confidence level | |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | The Culicidae was found to be negative for Wolbachia. |
Woodlouse
Common Water Strider (Aquarius remigis)
Water Strider
Water Strider
Water Skipper