Sample information
Picture	Photos by: Alex Ambron, Syed Rahman, Hope Sallit
Location
Collection date	01/02/2025
Captive / Cultivated?	Wild-caught
Group	Penn State Lehigh Valley
Observations	In the landscape
Putative identification	Arthropoda Insecta Odonata Coenagrionidae
Methods
Extraction kit	DNeasy (Qiagen) blood and tissue kit
DNA extraction location	Partial abdomen
Single or Duplex PCR	Single Reaction
Gel electrophoresis system	Standard electrophoresis system Bio-Rad
Buffer	TAE
DNA stain	SYBR Safe
Gel images
Protocol notes	Lane 1= Ladder
Results
Wolbachia presence	No
Confidence level	Medium
Explanation of confidence level
Wolbachia 16S sequence
Arthropod COI sequence
Summary	The Coenagrionidae was found to be negative for Wolbachia.