DEM-JP Order Blattodea

April 14, 2025

Sample information
Picture
Photos by: Jenaro Paredes
Location No map location data available.
Collection date 01/27/2025
Captive / Cultivated? Wild-caught
Group Georgia Southern University
Observations

Collected GSU Armstrong campus

Underneath trees, Shaded, near body of water

Used swift force

Gloved Hand method
Putative identification Arthropoda Insecta Blattodea

Methods
Extraction kit
DNA extraction location Whole arthropod
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain Other
Gel images
Protocol notes

DNA extraction kit of in-house reagents was used.

 

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

With the results from the Gel Electrophoresis experiment, I was able to declare my insect was not infected due to where it sat with its base pairs.
Wolbachia 16S sequence
Arthropod COI sequence
TATCTTCggagCTTGATCTGGaATAGTAGGGACATCTTTAAGAATATTAA TCCGAGCAGAACTAAAtctaccTGGTTCATTAATTGGAGATGATCAAATC TATAATGTTATTGTAACGGCTCACGCTTTTGTTATAATTTTCTTTATAGT TATGCCAATTTTAATTGGAGGATTTGGAAATTGATTAGTACCTTTAATAT TAGGTGCTCCTGATATAGCATTTCCTCGTATAAATAATATAAGTTTTTGA CTTTTACCTCCATCTCTATCTCTTCTATTAGCCAGTAGCCTTGTCGAAAG AGGAGCTGGGACTGGTTGAACCGTATACCCTCCCTTAGCTAGAGGAATTG CTCATGCTGGAGCTTCAGTAGATTTAGCTATTTTCTCATTACATCTTGCA GGTGTATCATCAATTTTAGGTGCCGTAAATTTTATTTCAACAATTATTAA CATGAAACCAATTAATATAAGTCCTGAACGAATTCCTTTATTTGTTTGGT CAGTAGGTATTACTGCATTATTATTATTATTATCCTTACCAGTTCTTGCA GGTGCAATTACAATATTATTAACAGACCGAAATTTAAATACTTCATTTTT TGACCCTGCAGGGGGAGGTGATCCTATTTTATATCAACATTTATTTTGAT TTTTTGGTCACC
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Blattodea was found to be negative for Wolbachia.
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