BRO-KJS Diptera

April 17, 2025

Sample information
Picture
Photos by: Keiara S
Location
Collection date 01/30/2025
Captive / Cultivated? Wild-caught
Group Georgia Southern University
Observations

01/30/2025
10:50 am
Outside the science building, near the university garden, Georgia Southern Armstrong campus
Bushes, Trees, and Straw
Sweeping net
Putative identification Arthropoda Insecta

Methods
Extraction kit
DNA extraction location Whole arthropod
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain Other
Gel images
Protocol notes

DNA extraction kit of in-house reagents was used

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

I know my insect does not have Wolbachia. I am 100% sure I did everything correctly.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Insecta was found to be negative for Wolbachia.
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