BRO-NIM Order Blattodea

April 17, 2025

Sample information
Picture
Photos by: Naia M.
Location
Collection date 01/30/2025
Captive / Cultivated? Wild-caught
Group Georgia Southern University
Observations

Collection Date: 1/30/2025

Collection Time: 10:30 am

Collection Method: Aerial/Sweep Net

Location of Collection: Georgia Southern University
Armstrong Campus – near
police station and greenhouse

Description of Habitat: Dry grass, wet mud under
logs, small brush area with
leaves
Putative identification Arthropoda Insecta Blattodea

Methods
Extraction kit
DNA extraction location Whole arthropod
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain Other
Gel images
Protocol notes

used DNA extraction kit of in-house reagents

Results
Wolbachia presence Yes
Confidence level High
Explanation of confidence level

40% of insects in the same order were positive for Wolbachia, and my results showed the same
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Blattodea was found to be postive for Wolbachia.
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