Sample information |
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| Picture |
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|---|---|
| Location | |
| Collection date | 01/01/2025 |
| Captive / Cultivated? | Wild-caught |
| Group | Georgia Southern University |
| Observations | caught with a sweep net in dry and dead matted grass field with an abundance of dead and rotted logs. Insect appeared as A green small insect with shell like outside |
| Putative identification | |
Methods |
|
| Extraction kit | |
| DNA extraction location | head and lower body |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | Standard electrophoresis system |
| Buffer | TAE |
| DNA stain | Other |
| Gel images |
|
| Protocol notes | DNA extraction kit of in-house reagents was used |
Results |
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| Wolbachia presence | No |
| Confidence level | Medium |
| Explanation of confidence level | I feel like it was pretty accurate but I am new at this and could have made some mistakes along the way |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
ATCTGATcaggAATAGTAGGAATAATATTAAGAATAATTATTCGAATTGA ACTAGCTcatccAGGACCATTTTTAACAAATGATCAATTATATAATGTAA TTGTAACATCACATGCATTTATTATAATTTTTTTCATAGTAATACCAATT ATAATTGGGGGGTTTGGTAACTGATTATTACCTCTAATAATTGGAGCACC AGATATAGCATTCCCACGAATAAATAATATAAGATTCTGACTTTTACCAC CATCACTAACACTTCTATTAGTTAGATCTATAGTAGAAATAGGAGCAGGA ACTGGTTGAACAGTATACCCACCATTATCATCTAATGCAGCACATTCAGG ACCTAGAGTTGACCTAGCAATTTTCTCACTACATTTAGCTGGTATTTCAT CAATCTTAGGAGCAGTTAATTTTATTACAACACTTATTAATATACGAAGT ATTGGTATAAATTTAGATCGAACACCATTATTTGCATGATCAGTTATAAT TACTGCAATTTTACTATTATTATCTCTACCAGTATTAGCTGGGGCAATTA CTATACTATTAACAGATCGTAATTTAAATACATCATTCTTTGACCCAGCT GGGGGTGGGGATCCTATTTTATATCAACATTTATTTTGATTTTTTGG
BLAST at The Wolbachia Project BLAST at NCBI
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| Summary | |
Common Eastern Bumble Bee (Bombus impatiens)
American Bird
Spotted crane fly
Wolbachia data
Meadow Katydid