NV 60 Fall Field Cricket

April 15, 2021

Sample information
Picture
Photos by: Udhay Nallapati
Location
Collection date 10/04/2020
Captive / Cultivated? Wild-caught
Group Neuqua Valley High School
Observations
  • Was near about 5-6 other field crickets
  • Moves every time a loud noise occurs
  • Hops, but does not cover much distance
  • Very well blended into the grass
  • Was near high grass by a pond
Putative identification Arthropoda Hexapoda Insecta Orthoptera

Methods
Extraction kit Puregene (Qiagen)
DNA extraction location
Single or Duplex PCR
Gel electrophoresis system Standard electrophoresis system
Buffer LB
DNA stain GelRed
Gel images
Protocol notes

Results
Wolbachia presence Yes
Confidence level High
Explanation of confidence level

The Gel shows that the Cricket was positive for Wolbachia.
Wolbachia 16S sequence Download AB1
Arthropod COI sequence Download AB1
Summary The Orthoptera was found to be postive for Wolbachia.
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