NV 80 House Cricket

Sample information

Picture
Photos by: Hallie B.
Location
Collection date 10/04/2020
Captive / Cultivated? Wild-caught
Group Neuqua Valley High School
Observations

Found on the floor of my garage, jumping fast and was aware

Putative identification Hexapoda Insecta Orthoptera Gryllidae Acheta Acheta domesticus

Methods

Extraction kit DNeasy (Qiagen)
DNA extraction location Abdomen
Gel electrophoresis system MiniPCR
Buffer TBE
DNA stain GelGreen
Gel images
Protocol notes

Results

Wolbachia presence No
Confidence level High
Explanation of confidence level

There is no band at all for our sample, it does not line up with the control.

Wolbachia 16S sequence
Arthropod COI sequence Download AB1
Summary The Acheta domesticus was found to be negative for Wolbachia.
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