NV 80 House Cricket

April 15, 2021

Sample information
Picture
Photos by: Hallie B.
Location
Collection date 10/04/2020
Captive / Cultivated? Wild-caught
Group Neuqua Valley High School
Observations

Found on the floor of my garage, jumping fast and was aware
Putative identification Arthropoda Hexapoda Insecta Orthoptera Gryllidae Acheta Acheta domesticus

Methods
Extraction kit DNeasy (Qiagen)
DNA extraction location Abdomen
Single or Duplex PCR
Gel electrophoresis system MiniPCR
Buffer TBE
DNA stain GelGreen
Gel images
Protocol notes

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

There is no band at all for our sample, it does not line up with the control.
Wolbachia 16S sequence
Arthropod COI sequence Download AB1
Summary The Acheta domesticus was found to be negative for Wolbachia.
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