Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 09/03/2024 |
| Captive / Cultivated? | Wild-caught |
| Group | Benedictine University |
| Observations | Small sized Bald Faced Hornet with yellow spotting across thorax and face with the overall insect a deep black color. Skinny waist with a pair of translucent wings. |
| Putative identification | Arthropoda Insecta Hymenoptera |
Methods |
|
| Extraction kit | DNeasy (Qiagen) blood and tissue kit |
| DNA extraction location | Whole arthropod |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | Standard electrophoresis system |
| Buffer | TAE |
| DNA stain | SYBR Safe |
| Gel images |
|
| Protocol notes | |
Results |
|
| Wolbachia presence | Unknown |
| Confidence level | High |
| Explanation of confidence level | Due to the fact that no arthropod DNA was received in my data and I had no DNA bands for arthropods, I was unable to see if my insect had Wolbachia or not. If I did get DNA I would look at the bands and see if my insect had Wolabachia but i was unable to do that due to the lack of presence of a band. The reason of no DNA could be due to errors in the lab such as now breaking down the insect enough during cell lysis or diluting the DNA too much during the Nanodrop lab. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | |
Common Eastern Bumble Bee (Bombus impatiens)
American Bird
Spotted crane fly
Wolbachia data
Meadow Katydid