Eastern Carpenter Bee

April 28, 2025

Sample information
Picture
Photos by: Lymaris M.
Location
Collection date 12/13/2030
Captive / Cultivated? Wild-caught
Group Longwood University
Observations
Putative identification

Methods
Extraction kit DNeasy (Qiagen)
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system MiniPCR
Buffer TAE
DNA stain Fast Blast
Gel images
Protocol notes

Results
Wolbachia presence No
Confidence level Medium
Explanation of confidence level

The controls worked as expected for my lab partner, however they did not work for my arthropod. Therefore, I cannot be confident that the arthropod was infected with Wolbachia
Wolbachia 16S sequence
Arthropod COI sequence
Summary
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