Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 05/09/2025 |
| Captive / Cultivated? | Wild-caught |
| Group | California Academy of Mathematics and Science |
| Observations | Insect was found crawling in grass when collected. |
| Putative identification | Arthropoda Insecta Coleoptera Coccinellidae Harmonia Harmonia axyridis |
Methods |
|
| Extraction kit | DNeasy (Qiagen) |
| DNA extraction location | Whole arthropod |
| Single or Duplex PCR | Duplex Reaction |
| Gel electrophoresis system | Standard electrophoresis system Bio-Rad |
| Buffer | 1X TAE |
| DNA stain | 100X Fast Blast |
| Gel images |
|
| Protocol notes | While a gel was run with Harmonia Axyridis samples/DNA gel electrophoresis was not run properly/ samples were not prepared correctly so no bands appeared on the gel. |
Results |
|
| Wolbachia presence | Unknown |
| Confidence level | High |
| Explanation of confidence level | No samples were submitted to be barcoded so it is unknown whether the Harmonia Axyridis contained Wolbachia. Additionally, during gel electrophoresis, no bands were seen for this sample including the wolbachia positive band and any other band proving PCR/DNA extraction was unsuccessful for this sample. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | |
Common Eastern Bumble Bee (Bombus impatiens)
American Bird
Spotted crane fly
Wolbachia data
Meadow Katydid