Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 09/11/2025 |
| Captive / Cultivated? | Wild-caught |
| Group | Penn State Lehigh Valley |
| Observations | |
| Putative identification | Arthropoda Arachnida Araneae |
Methods |
|
| Extraction kit | DNeasy (Qiagen) |
| DNA extraction location | Abdomen |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | Standard electrophoresis system |
| Buffer | 1X TAE |
| DNA stain | SYBR Green |
| Gel images |
|
| Protocol notes | For this DNA extraction, me and my lab partner followed the protocols listed on the wolbachia project website throughout the course of 4 weeks. We went out and collected our species, then they were frozen and we amplified their DNA after extraction from the abdomen. Using different solutions and buffers, we were able to isolate the DNA and using a gel electrophoresis system, we identified that this wolf spider was wolbachia positive. |
Results |
|
| Wolbachia presence | Yes |
| Confidence level | Low |
| Explanation of confidence level | We made a mistake and did not enter our DNA ladder, so we don’t have a basis to go off of |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | The Araneae was found to be postive for Wolbachia. |
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