Trochosa ruricola(Rustic Wolf Spider)

September 17, 2025

Sample information
Picture
Photos by: Kierra K
Location
Collection date 09/11/2025
Captive / Cultivated? Wild-caught
Group Penn State Lehigh Valley
Observations

Small and furry with a brown body. Two sections of body and light stripes. Has four eyes. Was hiding under a tree and kept trying to rush into the mulch around the trees when caught.
Putative identification Arthropoda Arachnida Araneae Lycosidae Trochosa Trochosa ruricola

Methods
Extraction kit Blood and Tissue kit
DNA extraction location Abdomen
Single or Duplex PCR
Gel electrophoresis system MiniPCR
Buffer TBE
DNA stain Cyber Stain
Gel images
Protocol notes

DNA extraction: I took just the abdomen of my specimen and made sure to crush it well in the tube before combining it with the difference chemicals.

Gel electrophoresis for CO1 arthropod PCR:

1 – DNA Ladder

2 – Spider specimen

3 – Bee specimen

4 – Wolbachia positive control

5 – Wolbachia negative control

6 – DNA control

7 – Water

Analysis: All of our wells seemed to take well and show up good. However, I don’t think the positive control is showing up correctly. This may be due to a mess up in the extraction process or loss of DNA.

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

During the process, the DNA ended up becoming loss or contaminated, causing the bands to not appear during electrophoresis.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Trochosa ruricola was found to be negative for Wolbachia.
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