Common Eastern Bumble Bee (Bombus impatiens)

September 11, 2025

Sample information
Picture
Photos by: Mariah Q.
Location
Collection date 09/11/2025
Captive / Cultivated? Wild-caught
Group Penn State Lehigh Valley
Observations

Found in a flower bush in a garden near a forested area.

Looks like a type of bee: 2 wings, 6 legs, 2 eyes, fuzzy yellow and black upper body, large smooth black lower body.
Putative identification Arthropoda Insecta Hymenoptera

Methods
Extraction kit Qiagen Blood and Tissue kit
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer 1X TAE
DNA stain Other
Gel images
Protocol notes

Lane 1: DNA Standard

Lane 2:  Arthropod #1 Wol + COI1 / MQAM03A+W1

Lane 3: Arthropod #2 Wol + COl1 / MQAM03A+W2

Lane 4: Wol Pos Wol + COl1 / MQAM03Pos

Lane 5: Wol Neg Wol + COl1 / MQAM03Neg

Lane 6: gDNA control Wol + COl1 / MQAM03DNAC

Lane 7: water control Wol + COl1 / MQAM03Water

Lane 8:  EMPTY

Lane 9: Arthropod #1 Wol + COI1 / SGGS03AtW1

Lane 10: Arthropod #2 Wol + COl1 / SGGS03AtW2

Lane 11: Wol Pos Wol + COl1 / SGGS03Pos

Lane 12: Wol Neg Wol + COl1 / SGGS03Neg

Lane 13: gDNA control Wol + COl1 / SGGS03DNA

Lane 14: water control Wol + COl1 / SGGS03Water

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

No band for Wolbachia on gel electrophoresis results.
Wolbachia 16S sequence
Arthropod COI sequence
TCGNATCATCAATAAGATTATTAATTCNAATANAACTTAGACATCCAGGA ATATGAATTAATAATGATCAAATTTATAATTCATTAGTTACAAGACATGCATTTTTAATAATTTTTTTTATAGTTATACC ATTTATAATTGGAGGATTCGGAAATTATTTAATTCCTTTAATATTAGGATCACCTGATATAGCTTTTCCACGAATAAATA ATATTAGATTTTGATTATTACCCCCATCTATTTTTATATTATTATTAAGAACTTTATTTACACCTAATGTAGGAACAGGT TGAACCGTATATCCCCCTTTATCATCTTATTTATTTCATTCATCACCTTCAGTAGATATTGCAATTTTTTCTTTACATGT AACAGGAATTTCTTCTATTATTGGTTCATTAAATTTTATCGTNGCTATTATATTAATAAAAAATTTTTCATTAAATTATG ATCAAATTACTTTATTTTCATGATCTGNATGTATTACAGTATTATTATTAATTTTATCATTACCAGTTTTAGCAGGAGCA ATTACTATACTTCTTTTTGATCGAAATTTTAATACATCATTTTTTGATCCAATAGGAGGTGGAGATCCAATTNNATATCA ACNTTTTATTTTGANNNTNTTGGTCACCCTGAANNTTAA
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Hymenoptera was found to be negative for Wolbachia.
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