Sample information |
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| Picture |
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|---|---|
| Location | |
| Collection date | 09/11/2025 |
| Captive / Cultivated? | Wild-caught |
| Group | Penn State Lehigh Valley |
| Observations |
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| Putative identification | Arthropoda |
Methods |
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| Extraction kit | DNeasy (Qiagen) |
| DNA extraction location | Abdomen |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | Standard electrophoresis system |
| Buffer | 1X TAE |
| DNA stain | SYBR Safe |
| Gel images |
|
| Protocol notes | |
Results |
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| Wolbachia presence | Yes |
| Confidence level | Medium |
| Explanation of confidence level | Medium because it’s my first time doing something like that and at the beginning I was a little confused and then the things worked well |
| Wolbachia 16S sequence |
NTTNNNNNNANNNGNNNNNGTCTGGTATCCTCTCAAAANTGAGATNNCGAGNAAAATCCNCNACCCT GGANCCNTNNTNNGGNGACATTTTTANTNNCTGTTGACTCTNCNCACGNGTTTTTAATANNTTTNTNAAAGGTNGGNATT ATGTNNTGCATGNGNNNCNANATGGANAGCCTACATCNTGAGGAGNGNTGGAAANNTGTGCCNCNAATANNNNATGAAAT NTNNGCCCTTNNNCCCATCTTAATTTTTNTTGTGCTCNNCAACANNTTTGAAAAAAGTAGTGNTACCAGGAAAACTGGAA TNCCCCCCTCATGNTNAATACTTNNNCGTGTCNGGACCTCNNTGCCCGTAACTCNTTTNTAATTGGATNTAGCAGGAANT NNTNCTATTTTAGGAGCTTTTAATTTTNTTACAACAATTATTAATATACGATTAAAAAGTTTACCTTTTGATCNATACCA TTATTTTNNGAGCAGAAGGAATTACAGCATTTTNTTTATTATTATCTTTACCTGTATTAGCTGGAGCTATTACAATATAA TAAACAGATCTAAATTTAAAAACTTCTTTTTTTTGACCCAGCTNNNNNANGGANNNCNNATNTTANTCNNNTTAATTNTG ATTTTTNNNNNNCAANNTCNNAA
BLAST at The Wolbachia Project BLAST at NCBI
|
| Arthropod COI sequence |
TGATCNGGAATAATNGGATCATCAATAAGATTATTAATTCGAATAGAACTTAGACATCCAGGAATA TGAATTAATAATGATCAAATTTATAATTCATTAGTTACTAGACATGCATTTTTAATAATTTTTTTTATAGTTATACCATT TATAATTGGAGGATTTGGAAATTATTTAATTCCTTTAATATTAGGATCACCTGATATAGCTTTCCCACGAATAAATAATA TTAGATTTTGATTATTACCTCCATCTATTTTTATATTATTATTAAGAACTTTATTTACACCTAATGTAGGAACAGGTTGA ACTGTATATCCCCCTTTATCATCTTATTTATTTCATTCATCACCTTCAGTAGATATTGCAATTTTTTCTTTACATATAAC AGGAATTTCTTCTATTATTGGTTCATTAAATTTTATTGTTACNATTATATTAATAAAAAATTTTTCATTAAATTATGATC AAATTACTTTATTTTCTTGATCTGTATGTATTACAGTNTTATTATTAATTTTATCATTACCAGTTTTAGCTGGAGCAATT ACTATACTTCTTTTTGATCGAAATTTTAATACATCATTTTTTGATCCAATAGGAGGTGGAGATCCAATTTTATATCAACA TTTATTTTGATTTTTTG
BLAST at The Wolbachia Project BLAST at NCBI
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| Summary | The Arthropoda was found to be postive for Wolbachia. |
Common Eastern Bumble Bee (Bombus impatiens)
American Bird
Spotted crane fly
Wolbachia data
Meadow Katydid