Sample information
Picture	Photos by: F. Broad
Location
Collection date	09/04/2025
Captive / Cultivated?	Wild-caught
Group	St. Albans School
Observations
Putative identification	Arthropoda Insecta Lepidoptera Hesperiidae Atalopedes Atalopedes campestris
Methods
Extraction kit	DNeasy (Qiagen)
DNA extraction location	Abdomen
Single or Duplex PCR	Single Reaction
Gel electrophoresis system	MiniPCR
Buffer	TBE
DNA stain	GelGreen
Gel images
Protocol notes
Results
Wolbachia presence	No
Confidence level	High
Explanation of confidence level	There was no band to show that there was Wolbachia.
Wolbachia 16S sequence
Arthropod COI sequence	Download FASTA
Summary	The Atalopedes campestris was found to be negative for Wolbachia.