Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 09/17/2025 |
| Captive / Cultivated? | Wild-caught |
| Group | St. Paul Academy and Summit School |
| Observations | Ant was black and was smaller than a grain of rice. It was found between cracks in a sidewalk. |
| Putative identification | Arthropoda Insecta Hymenoptera |
Methods |
|
| Extraction kit | DNeasy (Qiagen) blood and tissue kit |
| DNA extraction location | Whole arthropod |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | MiniPCR |
| Buffer | TBE |
| DNA stain | SeeGreen |
| Gel images |
|
| Protocol notes | My arthropod’s DNA was in lanes 6 and 7. Lane 6 contained AK1A and tested for arthropod DNA, and lane 7 contained AK1W and tested for Wolbachia DNA. |
Results |
|
| Wolbachia presence | No |
| Confidence level | High |
| Explanation of confidence level | The band in the gel for gel electrophoresis was a bright green hue for arthropod DNA, and there was no band for Wolbachia DNA. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence | Download AB1
|
| Summary | The Hymenoptera was found to be negative for Wolbachia. |
European Paper Wasp
Woodworm Ant