Sample information |
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| Picture |
|
|---|---|
| Location | |
| Collection date | 09/17/2025 |
| Captive / Cultivated? | Wild-caught |
| Group | St. Paul Academy and Summit School |
| Observations | Extremely small ant, found above the dirt between the sidewalk and the street, Brownish-red color. Prefer to nest in open habitats. Colonies are unwilling to accept other queens or merge with other colonies. Colonies have a nupital flight around Labor Day (September). |
| Putative identification | Arthropoda Insecta Hymenoptera Formicidae Lasius Lasius neoniger |
Methods |
|
| Extraction kit | Qiagen DNeasy Blood and Tissue Kit. |
| DNA extraction location | Whole arthropod |
| Single or Duplex PCR | Duplex Reaction |
| Gel electrophoresis system | MiniPCR |
| Buffer | TBE |
| DNA stain | SeeGreen |
| Gel images |
|
| Protocol notes | Lane 1: DNA Ladder. Lane 8: Ant Arthropod DNA. Lane 9: Ant Wolbachia DNA. |
Results |
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| Wolbachia presence | No |
| Confidence level | High |
| Explanation of confidence level | The controls worked, we used PCR to replicate the DNA many times, and there was no fluorescent band in Lane 9, indicating no presence of Wolbachia DNA in the ant. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence | Download AB1
|
| Summary | The Lasius neoniger was found to be negative for Wolbachia. |
Red-Legged Grasshopper
Woodworm Ant