Ant

May 6, 2021

Sample information
Picture
Location
Collection date 03/28/2021
Captive / Cultivated? Wild-caught
Group Hampton Roads Academy
Observations

My ant was located on a window seek near house plants. It had 2 antennas, 6 legs, and no wings.
Putative identification Arthropoda Hexapoda Insecta Hymenoptera

Methods
Extraction kit Edwards Buffer
DNA extraction location Whole arthropod
Single or Duplex PCR
Gel electrophoresis system MiniOne
Buffer TBE
DNA stain GelGreen
Gel images
Protocol notes

My pellet was small and white. When using the pipet to take out part of the supernatant, I disturbed the pellet. I had to redo this step.

Results
Wolbachia presence No
Confidence level Low
Explanation of confidence level

My confidence level is low because I cannot see a precise band. It appears to be more of a smear.

The band was faint. First time doing the experiment, not sure about the +A band.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Hymenoptera was found to be negative for Wolbachia.
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