Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 05/09/2021 |
| Captive / Cultivated? | Wild-caught |
| Group | Hampton Roads Academy |
| Observations | Black color, has legs, no wings |
| Putative identification | Arthropoda Insecta Coleoptera |
Methods |
|
| Extraction kit | Edwards Buffer |
| DNA extraction location | Whole arthropod |
| Single or Duplex PCR | |
| Gel electrophoresis system | MiniOne |
| Buffer | TBE |
| DNA stain | GelGreen |
| Gel images |
|
| Protocol notes | My lab went pretty well. My pellet had a very pale color and it was a small amount. I had no mess ups In my lab but I wasn’t there for the day they found the wolbachia. |
Results |
|
| Wolbachia presence | Unknown |
| Confidence level | Low |
| Explanation of confidence level | My confidence level is low because I wasn’t there for the part when they found the wolbachia and there wasn’t enough gel to run another time. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | |
Common Eastern Bumble Bee (Bombus impatiens)
American Bird
Spotted crane fly
Wolbachia data
Meadow Katydid