Beetle

May 6, 2021

Sample information
Picture
Location
Collection date 05/09/2021
Captive / Cultivated? Wild-caught
Group Hampton Roads Academy
Observations

Black color, has legs, no wings
Putative identification Arthropoda Hexapoda Insecta Coleoptera

Methods
Extraction kit Edwards Buffer
DNA extraction location Whole arthropod
Single or Duplex PCR
Gel electrophoresis system MiniOne
Buffer TBE
DNA stain GelGreen
Gel images
Protocol notes

My lab went pretty well. My pellet had a very pale color and it was a small amount. I had no mess ups In my lab but I wasn’t there for the day they found the wolbachia.

Results
Wolbachia presence Unknown
Confidence level Low
Explanation of confidence level

My confidence level is low because I wasn’t there for the part when they found the wolbachia and there wasn’t enough gel to run another time.
Wolbachia 16S sequence
Arthropod COI sequence
Summary
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