MAT-STW Hymenoptera

November 12, 2025

Sample information
Picture
Photos by: Skyla W.
Location No map location data available.
Collection date 08/27/2025
Captive / Cultivated? Wild-caught
Group Georgia Southern University
Observations

Collection Method- Gloves

Detailed description of the collection site habitat- Planted tree, bushes, pine straw

 
Putative identification Arthropoda Insecta Hymenoptera

Methods
Extraction kit
DNA extraction location head and lower body
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain Other
Gel images
Protocol notes

DNA extraction kit of in-house reagents was used.

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

I’m highly confident in my results because my controls worked and every aspect of the entire process went the way it was supposed to.
Wolbachia 16S sequence
Arthropod COI sequence
ATCATCTATAAGAATAATCATCCGATTAGAACTAGGATCTTGTAATTCTC TAATTAATAATGATCAAATCTACAACTCCTTAGTTACTAGACACGCTTTT ATTATAATTTTTTTTATAGTTATGCCCTTCATAATTGGAGGATTTGGAAA TTTCTTAGTGCCCTTAATACTTGGGTCCCCCGATATGGCCTATCCTCGAA TAAATAATATAAGATTTTGACTTCTGCCACCTTCTCTAACTCTTTTACTT ATCAGAAGATTTATTAACAGAGGAGTAGGAACAGGATGAACTATCTACCC ACCATTAGCCTCCAATATTTTTCACAGAGGGGCCTCTATTGATCTATCTA TTTTTTCCTTACATATCGCCGGAATATCATCAATTTTAGGAGCTATTAAT TTTATTTCTACAATCATTAACATACACCATAAAAATTTTACTATAGATAA AATCCCCCTACTAGTTTGATCTATCCTTATTACAGCCATTCTTCTTTTAC TTTCACTCCCAGTTCTTGCAGGAGCTATTACAATATTACTAACTGACCGT AATTTAAACACCTCCTTCTTCGATCCCTCAGGAGGAGGAGATCCCATTCT ATACCAACATTTATTCTGATTTTTTGGTCACC
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Hymenoptera was found to be negative for Wolbachia.
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