MAC-SPT

November 12, 2025

Sample information
Picture
Photos by: Symphynie T
Location
Collection date 08/28/2025
Captive / Cultivated? Wild-caught
Group Georgia Southern University
Observations

Was caught in grassy/dirt areas.
Putative identification Arthropoda Insecta Blattodea Ectobiidae Supella Supella longipalpa

Methods
Extraction kit
DNA extraction location Whole arthropod
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain Other
Gel images
Protocol notes

DNA extraction kit of in-house reagents was used.

Results
Wolbachia presence Yes
Confidence level High
Explanation of confidence level

High because everything in this process has gone well, and I can trust my results because I followed instructions carefully.
Wolbachia 16S sequence
>03D_MAC-SPT_CO1F TRIM QUALITY: 20 TGGATAGTAGgGACATCTTTAAGAATATTAATCCGAGCAGAACTAAATCA ACCTgttTCATTAATTGGAGATGATCAAATCTATAATGTTATTGTAACGG CTCACGCTTTTGTTATAATTTTCTTTATAGTTATGCCAATTTTAATTGGA GGATTTGGAAATTGATTAGTACCTTTAATATTAGGTGCTCCTGATATAGC ATTTCCTCGTATAAATAATATAAGTTTTTGACTTTTACCTCCATCTCTAT CTCTTCTATTAGCCAGTAGCCTTGTCGAAAGAGGAGCTGGGACTGGTTGA ACCGTATACCCTCCCTTAGCTAGAGGAATTGCTCATGCTGGAGCTTCAGT AGATTTAGCTATTTTCTCATTACATCTTGCAGGTGTATCATCAATTTTAG GTGCCGTAAATTTTATTTCAACAATTATTAACATGAAACCAATTAATATA AGCCCTGAACGAATTCCTTTATTTGTTTGGTCAGTAGGTATTACTGCATT ATTATTATTATTATCCTTACCAGTTCTTGCAGGTGCAATTACAATATTAT TAACAGACCGAAATTTAAATACTTCATTTTTTGACCCTGCAGGGGGAGGT GATCCTATTTTATATCAACATTTATTTTGATTTTTTGG
BLAST at The Wolbachia Project   BLAST at NCBI
Arthropod COI sequence
Summary The Supella longipalpa was found to be postive for Wolbachia.
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