Dermacentor Variabilis

November 13, 2025

Sample information
Picture
Photos by: Ken K.
Location
Collection date 11/07/2025
Captive / Cultivated? Wild-caught
Group Walton High School
Observations

The arthropod was small in size, approximately 2-3 millimeters in length. The arthropod was moving with 6-8 legs and was brown in body color.
Putative identification Arthropoda Arachnida Ixodida Ixodidae Dermacentor Dermacentor variabilis

Methods
Extraction kit DNeasy (Qiagen) blood and tissue kit
DNA extraction location Whole arthropod
Single or Duplex PCR Duplex Reaction
Gel electrophoresis system
Buffer
DNA stain
Gel images
Protocol notes

Involved grinding the arthropods to ensure a high DNA yield

Results
Wolbachia presence
Confidence level
Explanation of confidence level
Wolbachia 16S sequence
Arthropod COI sequence
Summary
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