Benedictine University Student-Chris R

November 18, 2025

Sample information
Picture
Photos by: Chris Rojas
Location
Collection date 09/02/2025
Captive / Cultivated? Wild-caught
Group Benedictine University
Observations

Found in a tall grass area, near the trees.
Putative identification Arthropoda Insecta Orthoptera

Methods
Extraction kit DNeasy (Qiagen) blood and tissue kit
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain SYBR Safe
Gel images
Protocol notes

Results
Wolbachia presence No
Confidence level Medium
Explanation of confidence level

I could’ve made an error and I also dropped some of the DNA when doing the lab.
Wolbachia 16S sequence
>w5R.ab1 (346 bp) GCTGATCCACGATTACTAGCGATTCCAACTTCATGCACTCGAGTTGCAGAGTACAATCCGAACTGAGATGGCTTTTAAGG GATTAGCTTAGCCTCGCGACTTTGCAGCCCATTGTAGCCACCATTGTAGCACGTGTGTAGCCCACTCCATAAGGGCCATG ATGACTTGACATCATCCCCACCTTCCTCCAGTTTATCACTGGCAGTTTCCTTAAAGTCCCCAGCATTACCTGATGGTAAC TAAGGATGAGGGTTGCGCTCGTTGCGGGACTT
BLAST at The Wolbachia Project   BLAST at NCBI
Arthropod COI sequence Download FASTA    Download AB1
>87-CA_Y1_CoR (584 bp) AGTATTCCATTTCGACAGTTAATAATATCGTAATCGCTTCGCTAATACAGGAAGCGAAATAATAAAAGGCGCGCCGTAAA GCTAACCGATCGACAGTAATGGCGTTGGTCAGTGTTACTCCCTTCGATCGTACACAATCGCTGTTGTAACAAAACCGACC GCCCCTAGAGTTGATGAAACACCAGCTAAGTGAAGCGAGAAAATGGCTAAATCAACTGACGCTCCCCATGTGCAATCGCT CCAGCGAGTGGGGGGTAAACTGTTCATCCTGTACCAGCTCCATTATCAACCATAGATGAGGTAAGTAAAAGGGTTAGTGA TGGTGGTAAAAGTCAAAAACTTATATTATTTATTCGTGGAAAAGCTATATCTGGTGCTCCAATTATTAATGGTACAAGTC AATTACCAAATCCTCCAATTATAATAGGTATTACTATAAAGAAAATTATTACAAATGCGTGGGCTGTAATAATTACATTA TAGATTTGGTCATCTCCAATTAGAGATCCTGGTTGTCCTAGTTCTGCTCGAATAATTATTCTTATAGAAGTTCCTACTAT TCCAGCTCATGCTCCAAATATAAA
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Orthoptera was found to be negative for Wolbachia.
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