Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 09/03/2025 |
| Captive / Cultivated? | Wild-caught |
| Group | Benedictine University |
| Observations | |
| Putative identification | Arthropoda |
Methods |
|
| Extraction kit | DNeasy (Qiagen) |
| DNA extraction location | Abdomen |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | Standard electrophoresis system |
| Buffer | 1X TAE |
| DNA stain | SYBR Safe |
| Gel images |
|
| Protocol notes | |
Results |
|
| Wolbachia presence | No |
| Confidence level | Medium |
| Explanation of confidence level | Results showed no Wolbachia (+) |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | The Arthropoda was found to be negative for Wolbachia. |


Melanoplus bivittatus – Xander Estacio
Bombus impatients (Eastern Bumblebee)
Agelenopis
Common Eastern Bumble Bee (Bombus impatiens)