Vespula

December 5, 2025

Sample information
Picture
Photos by: Manaar. M
Location
Collection date 09/02/2025
Captive / Cultivated? Wild-caught
Group Benedictine University
Observations

Collected by Fall 2025 students

Sample collected by killing jar

By the trees of the parking garage.

 
Putative identification Arthropoda

Methods
Extraction kit DNeasy (Qiagen) blood and tissue kit
DNA extraction location Whole arthropod
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain SYBR Safe
Gel images
Protocol notes

I inserted my DNA in the third column….. and I have a strong band of visible DNA.

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

There was no band at all for my wolbachia.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Arthropoda was found to be negative for Wolbachia.
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