5A – Greenhouse Millipede

January 7, 2026

Sample information
Picture
Entry by: Iris B
Location
Collection date 09/04/2025
Captive / Cultivated? Wild-caught
Group St. Paul Academy and Summit School
Observations

The millipede was found under a rock in a garden. It was collected in the afternoon on September 4th, the temperature was high 50s Fahrenheit. There was many other smaller insects under the rocks, but only one millipede was collected.
Putative identification Arthropoda Diplopoda Polydesmida

Methods
Extraction kit Qiagen DNeasy Blood and Tissue Kit
DNA extraction location Whole arthropod
Single or Duplex PCR Duplex Reaction
Gel electrophoresis system MiniPCR
Buffer TBE
DNA stain SeeGreen
Gel images
Protocol notes

Followed Wolbachia Project Protocols

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Polydesmida was found to be negative for Wolbachia.
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