Spider

January 25, 2026

Sample information
Picture
Entry by: Bayne T
Location
Collection date 09/08/2025
Captive / Cultivated? Wild-caught
Group St. Paul Academy and Summit School
Observations

Found near a wall in the cracks of a brick tile with sand, during the fall in 74 degree weather
Putative identification Arthropoda Arachnida Opiliones

Methods
Extraction kit pcr
DNA extraction location Whole arthropod
Single or Duplex PCR Single Reaction
Gel electrophoresis system agarose gel electrophoresis
Buffer
DNA stain
Gel images
Protocol notes

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

There is barely one line, so there is only a bit of DNA and not 2 lines, so there is no Wolbachia
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Opiliones was found to be negative for Wolbachia.
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