6G Pavement Ant Wolbachia Expirement

January 27, 2026

Sample information
Picture
Entry by: Sara K
Location
Collection date 09/08/2025
Captive / Cultivated? Wild-caught
Group St. Paul Academy and Summit School
Observations

Tiny and very small with 6 legs and
Putative identification Arthropoda Insecta Hymenoptera Formicidae

Methods
Extraction kit DNeasy (Qiagen) blood and tissue kit
DNA extraction location Whole arthropod
Single or Duplex PCR Duplex Reaction
Gel electrophoresis system MiniPCR
Buffer TBE
DNA stain SeaGreen
Gel images
Protocol notes

Followed Wolbachia Project Protocols

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

I am very confident in my results because there was one strand that matched up with the CO1 arthropod DNA on the ladder.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Formicidae was found to be negative for Wolbachia.
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