Sample information
Picture
Location
Collection date	09/09/2025
Captive / Cultivated?	Wild-caught
Group	St. Paul Academy and Summit School
Observations
Putative identification	Arthropoda Diplopoda Julida Julidae Ophyiulus Ophyiulus pilosus
Methods
Extraction kit	DNeasy (Qiagen)
DNA extraction location	Larva
Single or Duplex PCR	Single Reaction
Gel electrophoresis system	agarose gel electrophoresis
Buffer	TBE
DNA stain	Other
Gel images
Protocol notes
Results
Wolbachia presence	No
Confidence level	High
Explanation of confidence level	Lane 2 – 6A
Wolbachia 16S sequence
Arthropod COI sequence
Summary	The Ophyiulus pilosus was found to be negative for Wolbachia.