Sample information
Picture
Location
Collection date	09/08/2025
Captive / Cultivated?	Wild-caught
Group	St. Paul Academy and Summit School
Observations	Found in a grass field at school. I collected it around noon in sunny, clear weather.
Putative identification	Arthropoda Insecta Orthoptera Acrididae Melanoplus Melanoplus femurrubrum
Methods
Extraction kit	DNeasy (Qiagen) blood and tissue kit
DNA extraction location	Abdomen
Single or Duplex PCR	Single Reaction
Gel electrophoresis system	MiniPCR
Buffer	TBE
DNA stain	GelGreen
Gel images
Protocol notes
Results
Wolbachia presence	No
Confidence level	Medium
Explanation of confidence level
Wolbachia 16S sequence
Arthropod COI sequence	Download AB1
Summary	The Melanoplus femurrubrum was found to be negative for Wolbachia.