7G

February 11, 2026

Sample information
Picture
Entry by: Niko L
Location
Collection date
Captive / Cultivated? Wild-caught
Group St. Paul Academy and Summit School
Observations

Large, Ant, missing a leg. Specific Species unknown. Caught in grass under cottonwood trees on a cool day, 50-60 degrees Fahrenheit, ant colony near.
Putative identification Arthropoda Insecta Hymenoptera Formicidae Camponotus Camponotus pennsylvanicus

Methods
Extraction kit DNeasy (Qiagen) blood and tissue kit
DNA extraction location Abdomen
Single or Duplex PCR Duplex Reaction
Gel electrophoresis system MiniPCR
Buffer TBE
DNA stain SeeGreen
Gel images
Protocol notes

Followed Wolbachia Project Protocols

Results
Wolbachia presence Yes
Confidence level High
Explanation of confidence level

I am confident since the lines are clear, while there was no control, all the DNA had arthropod DNA, meaning all the DNA was correctly taken from the arthropod
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Camponotus pennsylvanicus was found to be postive for Wolbachia.
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