Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 11/04/2025 |
| Captive / Cultivated? | Wild-caught |
| Group | Hampshire Regional High School |
| Observations | Light tan with a greenish hue and light brown near the head. Very skinny and short in shape, and seemed to be about a millimeter wide. |
| Putative identification | Arthropoda Insecta Lepidoptera Geometridae |
Methods |
|
| Extraction kit | QIAGEN DNeasy kit |
| DNA extraction location | Whole arthropod |
| Single or Duplex PCR | Duplex Reaction |
| Gel electrophoresis system | Standard electrophoresis system |
| Buffer | 1X TAE |
| DNA stain | SYBR Safe |
| Gel images |
|
| Protocol notes | |
Results |
|
| Wolbachia presence | No |
| Confidence level | Medium |
| Explanation of confidence level | I did not verify my results with others, so I am not positive. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | The Geometridae was found to be negative for Wolbachia. |





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