Brookelynn Foote- Coccinellidae

January 29, 2026

Sample information
Picture
Entry by: Brookelynn Foote
Location
Collection date 11/09/2025
Captive / Cultivated? Captive / Cultivated
Group Hamburg Area High School
Observations

a small dome shaped beetle, thats typically red or orange with black spots
Putative identification Arthropoda Insecta Coleoptera Coccinellidae

Methods
Extraction kit DNeasy (Qiagen)
DNA extraction location Partial abdomen
Single or Duplex PCR Duplex Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TBG
DNA stain SYBR-SAFE
Gel images
Protocol notes

Results
Wolbachia presence No
Confidence level Medium
Explanation of confidence level

My results were not confirmed, but my partner and I both agreed that Wolbachia is not present.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Coccinellidae was found to be negative for Wolbachia.
Report Inappropriate Post