Sample information
Picture	Entry by: Rheeya Khatri
Location
Collection date	09/17/2025
Captive / Cultivated?	Wild-caught
Group	Woburn Memorial High School
Observations	Founded at a bottom of the tree Carries Wolbachia
Putative identification	Arthropoda
Methods
Extraction kit	DNeasy (Qiagen) blood and tissue kit.
DNA extraction location	Whole arthropod
Single or Duplex PCR	Single Reaction
Gel electrophoresis system	agarose gel electrophoresis
Buffer	TBE
DNA stain	GelGreen
Gel images
Protocol notes
Results
Wolbachia presence	Yes
Confidence level	High
Explanation of confidence level	The bands were vibrant and clear,
Wolbachia 16S sequence
Arthropod COI sequence
Summary	The Arthropoda was found to be postive for Wolbachia.