Cricket tested negative for Wolbachia at 01801

February 4, 2026

Sample information
Picture
Entry by: Lily G
Location
Collection date 09/17/2025
Captive / Cultivated? Wild-caught
Group Woburn Memorial High School
Observations

Orthoptera
Putative identification Arthropoda Insecta Orthoptera

Methods
Extraction kit Qiagen kit for blood and tissues.
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system agarose gel electrophoresis
Buffer 1X TAE
DNA stain GelGreen
Gel images
Protocol notes

Results
Wolbachia presence No
Confidence level Medium
Explanation of confidence level

My confidence level is not high, because me and my groups execution of this lab felt unorganized and rushed at times. However, Wolbachia is detected in lane 3, which is another students insects, which makes me feel slightly more confident.
Wolbachia 16S sequence
~438 bop
BLAST at The Wolbachia Project   BLAST at NCBI
Arthropod COI sequence
~708 bp
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Orthoptera was found to be negative for Wolbachia.
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