Brown Marmorated Stink Bug

May 12, 2026

Sample information
Picture
Entry by: Jazmin Gregory
Location
Approximate location: 42.62, -73.12
Collection date 01/25/2026
Captive / Cultivated? Wild-caught
Group Berkshire Community College
Observations

6 legs, 2 feelers, 2 wings
Putative identification Arthropoda Insecta Hemiptera Pentatomidae Halyomorpha Halyomorpha halys

Methods
Extraction kit Monarch DNA extraction (NEB)
DNA extraction location Abdomen
Single or Duplex PCR Duplex Reaction
Gel electrophoresis system agarose gel electrophoresis
Buffer 1X TAE
DNA stain
Gel images
Protocol notes

Results
Wolbachia presence No
Confidence level Medium
Explanation of confidence level

The controls worked perfectly. I would say the only reason to question the results is because another round of PCR may need to be done to know for sure that there is no Wolbachia present.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Halyomorpha halys was found to be negative for Wolbachia.
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