Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 12/12/2025 |
| Captive / Cultivated? | Wild-caught |
| Group | Coalinga High School |
| Observations | I found this spider outside during winter. It was chilly, around 11:00 AM. The spider was in a shady area on a wall by the door of the boys’ locker room, near the track. |
| Putative identification | Arthropoda Arachnida Araneae Pholcidae |
Methods |
|
| Extraction kit | DNeasy (Qiagen) |
| DNA extraction location | Abdomen |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | MiniOne |
| Buffer | TBE |
| DNA stain | GelGreen |
| Gel images |
|
| Protocol notes | Follow mini one protocol. |
Results |
|
| Wolbachia presence | No |
| Confidence level | Low |
| Explanation of confidence level | My confidence level is low that our arthropods (spider and fly) are not positive for Wolbachia. This is because a band appeared in the water lane, which indicates contamination. Since the negative control should not show any bands, this suggests that DNA may have been accidentally present. The contamination could have occurred if pipette tips were not changed between samples, if the wrong vial was used, or if a lane was accidentally skipped or mislabeled during pipetting, causing the water sample to be placed in a different lane. Because of this, the results are not fully reliable. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | The Pholcidae was found to be negative for Wolbachia. |
Parson Spider
Wasp
Caterpillar
Fruit Fly
Fly