Sample information

The spider is very small, and it is curled up like a ball. When caught it was crawling.

Methods

DNA extraction: I made sure to crush the spider really good. I cut into the abdomen and made sure there was no exoskeleton in my tube.

Gel electrophoresis lanes: Arthropod 

1: DNA ladder

2: Spider

3: Wasp

4: unknown

5: Unknown

6: Unknown

7: Unknown

Analysis: Arthropod- My controls did not work. In lanes 4-7 we were supposed to see three band but only 2 showed up. I think this is because we could have not crushed up the negative or positive control good enough. From not crushing the arthropod good enough the DNA couldn’t actually get extracted so no results could show.

Gel Electropphorisis : Wolbachia 

1: DNA ladder

2: Spider

3: Wasp

4: (+) control

5: DNA control

6:(-) control

7: water

Analysis: Wolbachia- My controls did not work! PCR was not done correctly because we got no band for DNA control.

Results

My confidence level is low because only one band showed up and it was our negative control, which should have not shown up so that means PCR didn’t work so its inconclusive.