Bee

March 25, 2026

Sample information
Picture
Entry by: Black Group
Location
Collection date 12/13/2025
Captive / Cultivated? Wild-caught
Group Coalinga High School
Observations

The bee was found in a backyard next to a pool after it had rained.
Putative identification Arthropoda Insecta Hymenoptera Apidae Apis Apis mellifera

Methods
Extraction kit DNeasy (Qiagen)
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system MiniOne
Buffer TBE
DNA stain GelGreen
Gel images
Protocol notes

Followed MiniOne Protocol

Lane 1: DNA Ladder

Lane 2: Sample 1, Grasshopper

Lane 3: Sample 2, Bee

Lane 4: Positive arthropod control

Lane 5: Negative arthropod control

Lane 6: DNA positive control

Lane 7: Water

 

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

Our confidence level is high because all of our controls came out the way they were supposed to come out. Then, when we tested the bee for arthropod DNA, we found a band. After we tested it for Wolbachia DNA, no band was detected. Because everything came out the way they were supposed to, we can confidently say that the bee doesn’t have Wolbachia.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Apis mellifera was found to be negative for Wolbachia.
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