Catipillar

Sample information

Picture
Entry by: Gennesis Gervacio
Location
Collection date 11/10/2025
Captive / Cultivated? Wild-caught
Group Coalinga High School
Observations

In my observations was that when it was alive the caterpillar was a green shade and then later on when it died it turned to a black color as if it had burned or if someone had toasted it alive.

Putative identification Arthropoda

Methods

Extraction kit DNeasy (Qiagen)
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system MiniOne
Buffer TBE
DNA stain GelGreen
Gel images
Protocol notes

Followed Mini one Protocol.

Results

Wolbachia presence Unknown
Confidence level Medium
Explanation of confidence level

My confidence level is on medium because I think we did something wrong when we were grinding the DNA and didn’t grind it long enough for it to actually work. Our caterpillar and positive control band did not show up for Wolbachia electrophoresis gel. This is why we are unsure if the caterpillar has Wolbachia because the positive control didn’t work.

Arthropod                  Wolbachia

Wolbachia 16S sequence
Arthropod COI sequence
Summary
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