Sample information |
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| Picture |
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|---|---|
| Location | |
| Collection date | 05/29/2026 |
| Captive / Cultivated? | Wild-caught |
| Group | Coalinga High School |
| Observations | My spider is a Black Widow. This spider was found by an older electricity box shriveled up. This black Widow was found almost dead as well. It also has 8 legs was kinda smaller as well. |
| Putative identification | Arthropoda |
Methods |
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| Extraction kit | DNeasy (Qiagen) |
| DNA extraction location | Abdomen |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | MiniOne |
| Buffer | TBE |
| DNA stain | GelGreen |
| Gel images |
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| Protocol notes | Pipetting-When my group and I were pipetting we had not changed some of our tips during some of this process. DNA extraction- When my group and I were doing the DNA extraction we started line 1 in lane 2 for our Arthropod gel. So we were a gel ahead. DNA extraction- We
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Results |
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| Wolbachia presence | |
| Confidence level | |
| Explanation of confidence level | |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
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| Summary | |
Spider
Spider
Bee
Mosquito
Roly Poly