Sample information

We noticed that our bee has lost its typical yellow color and is now more darker with a brownish tone.

Methods

DNA Extraction: We made sure to include our exoskeleton and to refill our ATL buffer onto our bee.

Gel electrophoresis lanes for CO1 arthropod PCR:

1. Our DNA ladder.

2.  The lane for our preying mantis.

3.  The lane for our bee.

4. Our positive arthropod control.

5. Our negative arthropod control.

6. And our positive DNA control.

Analysis: We had no problem in our DNA extraction, however we have accidentally mixed up our lanes, but other than that our results still came out as negative for wolbachia in our arthropods.

Results

I feel confident because my group and I have properly followed each step onto our experiment. We made sure to be careful with our equipment and we haven’t caused any mistakes as well. That’s how high my confidence level is at this moment knowing we haven’t done anything wrong.