Bee

March 25, 2026

Sample information
Picture
Entry by: James Bourdeau
Location
Collection date 03/04/2026
Captive / Cultivated? Wild-caught
Group Coalinga High School
Observations

The Arthropod was found mid to early December by James’ house
Putative identification Arthropoda

Methods
Extraction kit DNeasy (Qiagen)
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system MiniOne
Buffer TBE
DNA stain GelGreen
Gel images
Protocol notes

We are unable to know if our arthropods had Wolbachia because we weren’t able to do the Wolbachia PCR electrophoresis, but during the Arthropod electrophoresis, we got a Wolbachia band in our Positive control

Results
Wolbachia presence Unknown
Confidence level Medium
Explanation of confidence level

My confidence level is pretty medium because we don’t have the main step we need to figure out if our arthropods have Wolbachia, and the fact that we got a Wolbachia band during our Arthropod PCR electrophoresis
Wolbachia 16S sequence
Arthropod COI sequence
Summary
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