Roly Poly

March 25, 2026

Sample information
Picture
Entry by: Sofia Cruz
Location
Collection date 12/12/2025
Captive / Cultivated? Wild-caught
Group Coalinga High School
Observations

The roly poly was found outside the garden in the dirt.
Putative identification Arthropoda Malacostraca Isopoda Armadillidae Armadillidium Armadillidium vulgare

Methods
Extraction kit DNeasy (Qiagen)
DNA extraction location Abdomen/cut:partial
Single or Duplex PCR Single Reaction
Gel electrophoresis system MiniOne
Buffer TBE
DNA stain GelGreen
Gel images
Protocol notes

Followed MiniOne Protocol

Lane 1: DNA Ladder

Lane 2: Bee

Lane 3: Roly Poly

Lane 4: Positive control

Lane 5: Negative control

Lane 6: DNA control

Lane 7: Water

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

I’m really confidence in this experiment result because our pre lab result matched the end result. We had some mistakes, like adding 023 µl PCR tubes at first. Then we fixed it by adding 200µl PCR tubes. The Wolbachia brand did matched. So we can say that our roly poly has no Wolbachia infection.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Armadillidium vulgare was found to be negative for Wolbachia.
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