Bee

March 25, 2026

Sample information
Picture
Entry by: Daniel R
Location
Collection date 11/30/2025
Captive / Cultivated? Wild-caught
Group Coalinga High School
Observations

I found this bee in a garden at my house, the bee was flying around some tiny sunflowers that I have. I caught it around 5 pm, and it was cloudy and about 68 degrees f outside. There were many bees in the garden, but I chose the fastest one because I liked having a challenge when catching it.
Putative identification Arthropoda Insecta Hymenoptera

Methods
Extraction kit DNeasy (Qiagen)
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system MiniOne
Buffer TBE
DNA stain GelGreen
Gel images
Protocol notes

followed mini one protocol

Lane one- DNA LADDER

Lane two-BEE

Lane Three- ROLLYPOLLY

Lane Four- POS DNA CONTROL

Lane Five- NEG DNA CONTROL

Lane Six- POS DNA CONTROL

Lane Seven- WATER

Results
Wolbachia presence No
Confidence level Medium
Explanation of confidence level

Our confidence level for the bee are in the middle, we do not have high confidence nor low confidence. We did have one mistake during the process of trying to check the bee for wolbachia, but we believe that we overcame the issue and got the right results.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Hymenoptera was found to be negative for Wolbachia.
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