Mosquito

March 25, 2026

Sample information
Picture
Entry by: Isla F.
Location
Collection date 12/03/2025
Captive / Cultivated? Wild-caught
Group Coalinga High School
Observations

It has two long skinny legs, it is small and has a blackish brown color to it. It has two wings attached.
Putative identification Arthropoda

Methods
Extraction kit DNeasy (Qiagen)
DNA extraction location Partial abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system MiniOne
Buffer TBE
DNA stain GelGreen
Gel images
Protocol notes

Followed Mini one protocol.

Results
Wolbachia presence Unknown
Confidence level Low
Explanation of confidence level

The controls did not work. At the start of it a few bands sightly showed up but, during the process all of the bands were gone. Therefore, I am not confident or sure my arthropod has Wolbachia since my results came in inconclusive.
Wolbachia 16S sequence
Arthropod COI sequence
Summary
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